Job Information
University of Washington RESEARCH SCIENTIST/ENGINEER 3 in Seattle, Washington
Req #: 233665
Department: DEPARTMENT OF NEUROLOGY
Posting Date: 05/08/2024
Closing Info: Open Until Filled
Salary: $5427 - $7,100 per month
Shift: First Shift
Notes: As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here. (https://hr.uw.edu/benefits/wp-content/uploads/sites/3/2018/02/benefits-professional-staff-librarians-academic-staff-20230701_a11y.pdf )
As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem-solving skills and dedication to build stronger minds and a healthier world.
UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty.
This is a grant-supported research scientist position supervised by Dr. Jeffrey Carroll. Research in the Carroll lab is primarily focused on enhancing treatments for people impacted by rare neurodegenerative diseases, including Huntington’s disease. The Carroll lab relies on cell and mouse model systems in an attempt to help understand the basic pathological mechanisms of disease, as well as test experimental therapeutics.
In this position, the Research Scientist will assist in conducting grant-sponsored research into the consequences of Huntingtin lowering in mouse and cell models of Huntington’s Disease. They will conduct hands-on research while also playing an important role in project management, data analysis, and drafting manuscripts describing our findings.
DUTIES AND RESPONSIBILITIES The Research Scientist/Engineer 3 (E S UAW RSE) will have the following responsibilities: Mouse Work (40%)
Assisting in maintaining the mouse colony
Sacrifice via intraperitoneal or subcutaneous injection of anesthetics and preparation of plasma samples
Dissection of tissues, including micro-dissection of brain tissue into sub-regions
Isolation of biomolecules (DNA, RNA and Protein) from tissue and cell samples
Treatment of mice via intraperitoneal, intravenous and subcutaneous injections
Injection of oliogonucleotide drugs intracerebroventricularly
Transcardial perfusion of mice with PBS and fixatives to generated fixed mouse tissues for histological analysisCell and Molecular Biology (40%)
Isolation of biomolecules (DNA, RNA and Protein) from tissue and cell samples
Quantification of target gene expression with quantitative real time PCR (QRT-PCR) or related techniques
Quantification of target protein expression with western blots
Staining of tissue slides for immunohistochemistry
Analysis of immunohistochemical images using existing protocols in the ImageJ software package
Significant experience with cloning plasmid DNA, including propagation of plasmids in E. coli, sub-cloning
Generation and validation of guide RNAs for CRISPR/Cas9 experiments
Sub-cloning guide-RNA and other cassettes into AAV-generation plasmids
Gene expression using RNASequencing techniques
Assays in immortalized cell culture including post-stessor viability, sub-cellular trafficking and enzyme activation assays.Analyzing Results and Writing Manuscripts (20%)
Reads and understands the literature around the topics of research
Generates research outputs in the forms of slide decks, manuscript text and figures, progress reports to funding agencies, conference posters and presentations
Careful record keeping using Microsoft Office and Cloud Based online Storage
Statistical analysis of data using R
Analysis of microscopy images using ImageJ and other programs MINIMUM REQUIREMENTS
Bachelor's degree in Engineering or Scientific Discipline
Four years of relevant experience, OR Equivalent combination of education/experience. Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration. ADDITIONAL REQUIREMENTS
Experience with microscopy (light, immunofluorescence and/or confocal)
Significant molecular biology experience
Significant cell culture experience
Significant experience with rodents in research, preferably in neuroscience research DESIRED QUALIFICATIONS
Significant experience in analyzing genome-wide transcriptional datasets
Experience with the R programming language
Mouse Behavioral analyses
Recovery surgeries in mice, including stereotaxic injections in rodent models
Experience with primary mammalian cell cultures and/or iPSC cultures
Experience with live-cell microscopy
Graduate degree in related field Application Process: The application process may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment, you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are access ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.
University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to, among other things, race, religion, color, national origin, sexual orientation, gender identity, sex, age, protected veteran or disabled status, or genetic information.
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