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University of Rochester Staff Scientist, SMD - 229144 in Rochester, New York

Staff Scientist, SMD Job ID 229144

Location School of Medicine & Dentistry Full/Part Time Full-Time Favorite Job Regular/Temporary Regular Opening

Full Time 40 hours Grade 055 Pediatrics M&D Neonatology


9:00 AM - 5:00 PM; Weekends/Rare Holidays


Position Summary:

Under general direction and with considerable latitude for exercise of independent judgment, provides direct research and research training support at the professional level by directing and executing small, complex research projects or major aspects of larger research projects; and provides extensive guidance to and/or directly supervises technicians, laboratory aides, graduate students, undergraduate students, postdoctoral appointees and professional staff. The successful applicant will work as a member of the Pryhuber laboratory team, as well as with members of the NIH collaboratives with whom the Pryhuber lab works. In addition, this role will work with faculty, students and technicians from multiple Departments throughout the University of Rochester Medical Center, including the Flow and Genomics Cores. The Staff Scientist will perform advanced cellular and molecular biology techniques, primarily to support the work of the Lung Molecular Atlas Program (LungMAP). This work requires human lung tissue dissociation and detailed characterization of the isolated lung cells by multiplexed flow cytometry/CyTOF. Importantly, the Scientist should have the skills and expectations to assist heavily with data analysis, manuscript and grant application preparation.

Supervision and Direction Provision:

This position will report directly to Dr. Pryhuber, Professor in Pediatrics and Environmental Medicine, and will also work closely with the laboratory and bioinformatics staff, to learn procedures and achieve the goals of the laboratory. The Laboratory Manager will supervise and give direction in the absence of Dr. Pryhuber.

Responsibilities / Standards:

Works with team to dissociate human lung tissue into isolated single cells, keeping accurate notes on procedures and results for each case. Demonstrates flexibility to meet the needs of the lung processing team. Assists with organization and scheduling of the processing team.

Develops multi-channel flow, image and sorting-cytometric assays and other appropriate assessments of isolated human lung cells. Applies the assays as quality assessments and investigative analyses of the cell preparations. Works closely with the Genomics and Flow Cytometry Cores to optimize and standardize these techniques. Prepares and accomplishes analysis of this data for review with PI, for publication and presentation.

Works with the GRC to develop single cell transcriptomics analyses, including cell preparation and preparation of resulting data for analysis, presentation and dataset release to scientific community.

Assists substantially with data analysis, presentation, grant and manuscript preparation.

Works with intramural and extramural collaborators in pulmonary biology and immunology in support of the LungMAP Human Tissue Core responsibilities as well as development of Pryhuber lab scientific goals.

Teaches students and laboratory co-workers the skills stated above.

Required Competencies:

Specific skills necessary include advanced knowledge of general and human sample laboratory safety processes, universal precautions and aseptic cell culture technique. Additional skills used on a routine basis include flow and imaging cytometry, qPCR, ELISA, tissue cell dissociation and cell culture, genomic and transcriptomic techniques and analysis, nucleotide and protein gels and imaging. Detailed documentation of work and results will be kept in a laboratory notebook, in written worksheets and/or electronic database formats as directed by PI. All raw data will be kept in an organized fashion in formats accessible to the PI and collaborating laboratories.

The Staff Scientist will maintain communication and earn the trust of collaborators and members of the laboratory, interacting and effectively communicating with staff internal and external to the Department and Institution, as guided by Dr. Pryhuber. He/she will perform literature searches and update PI and laboratory personnel on techniques relevant to LungMAP, Research Center findings and interactions, and make recommendations regarding future direction, especially as related to the LungMAP. He/she will participate in the preparation of publications as a co-author, meticulously editing drafts.

Also needed are flexibility in contributing to the LungMAP requirements of the lab, which can be unpredictable and require some night and weekend work. Also, efficiency in prioritizing and organizing assignments, skill in proactively resolving problems, improving experimental protocols, collaborating with colleagues, and recommending quality improvement. A high level of attention to detail and follow through is also needed; exercising independent judgment including, completing analyses and providing deliverables to collaborating laboratories will be expected. Must have reliable/efficient transportation. Travel to scientific conferences, meetings and the like may be required.


Doctoral degree with major course work in the field of assignment and a minimum of 2-5 years of relevant postdoctoral training. Evidence of outstanding accomplishments and motivation to support the laboratory’s studies of immunologic and pulmonary diseases of newborns. Effective data preparation, presentation and writing skills are expected, as is efficiency, productivity and effective communication and collaboration with co-workers

Machines And Equipment:

Proper use of advanced laboratory equipment and reagents is anticipated, including antibodies and enzymes, autoclave, liquid nitrogen/air/oxygen tanks, biosafety hoods, centrifuges, microscopes (light and fluorescent), thermocyclers, tissue culture equipment, flow cytometers, ELISA plate reader, spectrophotometer, fluorescence plate reader, gel electrophoresis, UV spectrophotometer, desktop computers (Mac/PC) and software including Microsoft Office, Excel, LDMS, BLIS, REDcap and PRISM.

NOTE: This document describes typical duties and responsibilities and is not intended to limit management from assigning other work as required. The position will also be rated according to attendance record.

How To Apply

All applicants must apply online.

EOE Minorities/Females/Protected Veterans/Disabled