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Apply for Job Job ID362057 LocationTwin Cities Job FamilyResearch-Researchers Full/Part TimeFull-Time Regular/TemporaryRegular Job Code8352R2 Employee ClassCivil Service Add to Favorite Jobs Email this Job About the Job This Researcher 2 employee will perform and independently interpret biological experiments with mouse models and human trophoblast stem cells, supervised by PI. Will help maintain mouse lines by setting up breeding cages and separating and genotyping offspring. Will set up timed mattings and perform dissections to collect tissues for imaging and molecular studies of in vivo placental development. Will use sterile tissue culture methods to maintain human placenta stem cells, generate knock-out and knock-in clonal lines and analyze the differentiation of stem cells using 3D culturing techniques to generate key cell types of the placenta in vitro. Will use molecular techniques such as genotyping, endpoint PCR, ELISA, western blot and qRT-PCR as well as immunofluorescence microscopy and live cell imaging to characterize differentiated cell types. Will prepare samples for genome-wide analysis using next-generation sequencing. Will maintain a laboratory notebook and update shared laboratory protocols and ensure adherence to regulatory policies of the University. Responsibilities: 15% - Oversee the use and maintenance of laboratory equipment, training personnel/volunteers, and stocking/ordering of consumable lab supplies. 25% - Oversee multiple tissue culture lines using sterile techniques. Perform lentiviral transduction and transient transfections, isolate clonal cell lines and extract DNA and RNA to identify lines of interest. 20% - Perform immunostaining and fluorescence microscopy of formaldehyde fixed tissues and cells. Optimize antibody staining conditions and acquire multicolor high resolution images using a fluorescence microscope. Maintain a repository of collected images and associated metadata. 20% - Help oversee a mouse colony by setting up breeding cages, weaning, genotyping, and maintaining records in a centralized database. Timed matings and dissection will also be used to assess the embryonic development of the mouse placenta from embryonic day 6.5 to 14.5. 10% - Perform general molecular biology assays including genotyping, endpoint PCR, qRT-PCR, gel electrophoresis, western blotting, cloning and DNA plasmid preparation. 10% - Support detailed records of all experiments and procedures in laboratory notebooks and in online databases and shared folders. Maintain and update shared laboratory protocols. Qualifications Required Qualifications: BS/BA and 1 years of related experience or equivalent combination a combination of education and work experience to equal five years. Knowledge of molecular biology techniques and molecular genetics is required. Applicant must also have excellent organizational and communication skills and be highly self-motivated. Benefits Working at the University At the University of Minnesota, you'll find a flexible work environment and supportive colleagues who are interested in lifelong learning. We prioritize work-life balance, allowing you to invest in the future of your career and in your life outside of work. The University also offers a comprehensive benefits package that includes: Competitive wages, paid holidays, and generous time off Continuous learning opportunities through professional training and degree-seeking programs supported by the Regents Tuition Benefit Program Low-cost medical, dental, and pharmacy plans Healthcare and dependent care flexible spending accounts University HSA contributions Disability and employer-paid life insurance Employee wellbeing program Excellent retirement plans with employer contribution Public Service Loan Forgiveness (PSLF) opportunity Financial counseling services Employee Assistance Program with eight sessions of counseling at no cost Please visit the Office of Human Resources website for more information regarding benefits. How To Apply Applications must be submitted online. To be considered for this position, please click the Apply button and follow the instructions. You will be given the opportunity to complete an online application for the position and attach a cover letter and resume. Additional documents may be attached after application by accessing your "My Job Applications" page and uploading documents in the "My Cover Letters and Attachments" section. To request an accommodation during the application process, please e-mail employ@umn.edu or call (612) 624-8647. Diversity Employment Requirements Any offer of employment is contingent upon the successful completion of a background check. Our presumption is that prospective employees are eligible to work here. Criminal convictions do not automatically disqualify finalists from employment. About the U of M The University of Minnesota, Twin Cities (UMTC) The University of Minnesota, Twin Cities (UMTC), is among the largest public research universities in the country, offering undergraduate, graduate, and professional students a multitude of opportunities for study and research. Located at the heart of one of the nation's most vibrant, diverse metropolitan communities, students on the campuses in Minneapolis and St. Paul benefit from extensive partnerships with world-renowned health centers, international corporations, government agencies, and arts, nonprofit, and public service organizations. At the University of Minnesota, we are proud to be recognized by the Star Tribune as a Top Workplace for 2021, as well as by Forbes as Best Employers for Women and one of America's Best Employers (2015, 2018, 2019, 2023), Best Employer for Diversity (2019, 2020), Best Employer for New Grads (2018, 2019), and Best Employer by State (2019, 2022). The University of Minnesota Medical School Committed to innovation and diversity, the Medical School educates physicians, scientists, and health professionals; generates knowledge and treatments; and cares for patients and communities with compassion and respect. We value excellence, inclusiveness, collaboration, and discovery. The mission of the regional campus located in Duluth is to be a national leader in improving healthcare access and outcomes in rural Minnesota and American Indian/Alaska Native (AI/AN) communities. In partnership with CentraCare, the regional campus in St. Cloud offers a wide range of patient experiences throughout students' education in Greater Minnesota and prepares them to become exceptional clinicians and leaders for rural and immigrant populations. Founded in 1888, the University of Minnesota Medical School has three campuses. A four-year MD program and the MD/PhD program are located on the Twin Cities campus in addition to MD programs at regional campuses in Duluth and St. Cloud. The University recognizes and values the importance of diversity and inclusion in enriching the employment experience of its employees and in supporting the academic mission. The University is committed to attracting and retaining employees with varying identities and backgrounds. The University of Minnesota provides equal access to and opportunity in its programs, facilities, and employment without regard to race, color, creed, religion, national origin, gender, age, marital status, disability, public assistance status, veteran status, sexual orientation, gender identity, or gender expression. To learn more about diversity at the U: http://diversity.umn.edu.